Rothera's test Procedure - Test Principle, Uses, Preparation and Interpretation - Ketone Bodies

rothera's test procedure, Principle, Uses, reagents Preparation and results Interpretation with normal reference range - test for ketone bodies. Principle of Rothera’s Test Acetoacetic acid and acetone react with alkaline solution of sodium nitroprusside to form a purple colored complex. This method can detect above 1-5 mg/dl of acetoacetic acid and 10-20 mg/dl of acetone. Beta-hydroxybutyrate is not detected. What is Rothera's test? Why do ketone levels rise in plasma and urine? Is Ketonuria a sign of dehydration? Can you have ketones without having diabetes? More Information: http://laboratorytests.org/rotheras-test/ Credits: Labs for life, a partnership project of MoHFW and CDC https://www.youtube.com/channel/UCGCm7p6yg20dH3pJ8yzPNTQ